Summary

In this study, the constituents of rhizome oils were analysed using gas chromatography-mass spectrometry. There are 25 compounds in Kaempferia rotunda L. tuberous rhizome: alpha-pinene (4,48%), camphene (20,85%), pentadecane (15,47%), camphor (10,15%), alpha terpinolene (1,01%), bornyl acetate (5,65%), alpha-selinene (2,32%) gamma-curcumene (3,22%, heptadecane (3,80%), alpha-cedrene (3,64%), alpha-amorphene (4,92%), alpha-curcumine (2,68%), benzyl-benzoate (7,56%), eucalypyol (1,01%) and some important other compounds. The protocol for in vitro propagation was conducted and study on chemical components of rhizomes from Kaempferia rotunda L. The MS medium supplemented with BA 2 mg/L and Kinetin 0,2 mg/L gave higher number of shoots (4,67 shoots/explant). MS 1/2 medium supplemented with NAA 0,5 mg/L was suitable for plantlet formation (shoot height : 12,05 cm and 14,56 roots/explant) . In vitro plant with complete vertion grown on substrates land reached 100% survival rate.